ssr_primer
Primer Details for TRINITY_DN84903_c1_g3_i6 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTCGTTTCCTGTGTGCAC | TCTTGCGCTCACCCTTCATT | Start: 2045 |
| Tm | 59.972 | 59.963 | End: 2261 |
| Size | 20 | 20 | 217 |
| Primer 2 details |
|---|
| Sequence | TGCCCCGGTGGAACTATAGA | AGAGACGGCCAAGTTCATGG | Start: 293 |
| Tm | 60.032 | 60.036 | End: 510 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TGCCCCGGTGGAACTATAGA | GGATCCCAGCTTTGTGACGA | Start: 293 |
| Tm | 60.032 | 60.036 | End: 428 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | TGCCCCGGTGGAACTATAGA | GAGACGGCCAAGTTCATGGA | Start: 293 |
| Tm | 60.032 | 60.036 | End: 509 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | CCATGAACTTGGCCGTCTCT | AGAGGAATATGAGGCGTGCG | Start: 491 |
| Tm | 60.036 | 59.967 | End: 681 |
| Size | 20 | 20 | 191 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTCGTTTCCTGTGTGCAC | TCTTGCGCTCACCCTTCATT | Start: 2045 |
| Tm | 59.972 | 59.963 | End: 2261 |
| Size | 20 | 20 | 217 |
| Primer 2 details |
|---|
| Sequence | TGCCCCGGTGGAACTATAGA | AGAGACGGCCAAGTTCATGG | Start: 293 |
| Tm | 60.032 | 60.036 | End: 510 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TGCCCCGGTGGAACTATAGA | GGATCCCAGCTTTGTGACGA | Start: 293 |
| Tm | 60.032 | 60.036 | End: 428 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | TGCCCCGGTGGAACTATAGA | GAGACGGCCAAGTTCATGGA | Start: 293 |
| Tm | 60.032 | 60.036 | End: 509 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | CCATGAACTTGGCCGTCTCT | AGAGGAATATGAGGCGTGCG | Start: 491 |
| Tm | 60.036 | 59.967 | End: 681 |
| Size | 20 | 20 | 191 |
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