ssr_primer
Primer Details for TRINITY_DN84888_c0_g2_i13 |
|---|
| (T)40: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTAACAGCGCGCCAGTC | CCCTGAGTCGCTGCATAGAG | Start: 47 |
| Tm | 60.041 | 59.968 | End: 151 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | TTTTTAACAGCGCGCCAGTC | GTTATCCCTGAGTCGCTGCA | Start: 47 |
| Tm | 60.041 | 59.824 | End: 156 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | CTCTATGCAGCGACTCAGGG | GCTTGAAATATGCCGGGCAC | Start: 132 |
| Tm | 59.968 | 60.249 | End: 376 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | CTCTATGCAGCGACTCAGGG | ACCGTGAAGGCGCTTTACAT | Start: 132 |
| Tm | 59.968 | 60.321 | End: 358 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | CTCTATGCAGCGACTCAGGG | GTTGTTTGATCCCCCTCCCC | Start: 132 |
| Tm | 59.968 | 60.324 | End: 288 |
| Size | 20 | 20 | 157 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTAACAGCGCGCCAGTC | CCCTGAGTCGCTGCATAGAG | Start: 47 |
| Tm | 60.041 | 59.968 | End: 151 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | TTTTTAACAGCGCGCCAGTC | GTTATCCCTGAGTCGCTGCA | Start: 47 |
| Tm | 60.041 | 59.824 | End: 156 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | CTCTATGCAGCGACTCAGGG | GCTTGAAATATGCCGGGCAC | Start: 132 |
| Tm | 59.968 | 60.249 | End: 376 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | CTCTATGCAGCGACTCAGGG | ACCGTGAAGGCGCTTTACAT | Start: 132 |
| Tm | 59.968 | 60.321 | End: 358 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | CTCTATGCAGCGACTCAGGG | GTTGTTTGATCCCCCTCCCC | Start: 132 |
| Tm | 59.968 | 60.324 | End: 288 |
| Size | 20 | 20 | 157 |
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