ssr_primer
Primer Details for TRINITY_DN84869_c5_g1_i5 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAGGACTTACGTAAGCCG | ACAACATGAAAAGTGGCGGC | Start: 1448 |
| Tm | 59.898 | 59.968 | End: 1608 |
| Size | 20 | 20 | 161 |
| Primer 2 details |
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| Sequence | GGGAGGACTTACGTAAGCCG | ATGAAAAGTGGCGGCTGAGA | Start: 1448 |
| Tm | 59.898 | 59.963 | End: 1603 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
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| Sequence | CGGTTTGGCTTCCCGAGTAT | TCCACCCCGATAAAGGAGGT | Start: 15 |
| Tm | 60.108 | 59.956 | End: 181 |
| Size | 20 | 20 | 167 |
| Primer 4 details |
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| Sequence | GGGAGGACTTACGTAAGCCG | GGCAAAGTCAAGTAAGCGCC | Start: 1448 |
| Tm | 59.898 | 60.11 | End: 1583 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
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| Sequence | GCCGCCACTTTTCATGTTGT | ACTACTTGTGTTGCCAGGCA | Start: 1589 |
| Tm | 59.968 | 59.817 | End: 1800 |
| Size | 20 | 20 | 212 |
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