ssr_primer
Primer Details for TRINITY_DN84869_c4_g1_i6 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCAGCGCAGTTTTTCAGAA | ACGCTCCAACGCTTTTTCAC | Start: 3611 |
| Tm | 59.969 | 59.971 | End: 3774 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
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| Sequence | GGAAGGAGGGAAAATGGGGG | TTCTGAAAAACTGCGCTGCC | Start: 3474 |
| Tm | 60.032 | 59.969 | End: 3630 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
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| Sequence | GGGAAGGAGGGAAAATGGGG | TTCTGAAAAACTGCGCTGCC | Start: 3473 |
| Tm | 60.032 | 59.969 | End: 3630 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
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| Sequence | GGGAAAATGGGGGAGATGGG | TTCTGAAAAACTGCGCTGCC | Start: 3481 |
| Tm | 60.106 | 59.969 | End: 3630 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
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| Sequence | CTCTCTTGAAGGCTGTGGCA | CTGCGTACTGGGCTACGAAT | Start: 2749 |
| Tm | 59.964 | 59.898 | End: 2902 |
| Size | 20 | 20 | 154 |
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