ssr_primer
Primer Details for TRINITY_DN84829_c5_g1_i2 |
|---|
| (A)30: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTAACGGCGCGGTCTACAT | ACGGCTTGCGTAACGTCATA | Start: 439 |
| Tm | 59.896 | 60.109 | End: 603 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | GTTCTTTTCCGCCAAACGCT | AACACGACTCCATTTCGCCA | Start: 131 |
| Tm | 59.97 | 60.25 | End: 230 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | GAGACTCGGGCTGAAGTGAG | ACGGCTTGCGTAACGTCATA | Start: 472 |
| Tm | 59.828 | 60.109 | End: 603 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | TGGCGAAATGGAGTCGTGTT | ATGTAGACCGCGCCGTTAAT | Start: 211 |
| Tm | 60.25 | 59.896 | End: 458 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | TTGGCGAAATGGAGTCGTGT | ATGTAGACCGCGCCGTTAAT | Start: 210 |
| Tm | 60.25 | 59.896 | End: 458 |
| Size | 20 | 20 | 249 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTAACGGCGCGGTCTACAT | ACGGCTTGCGTAACGTCATA | Start: 439 |
| Tm | 59.896 | 60.109 | End: 603 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | GTTCTTTTCCGCCAAACGCT | AACACGACTCCATTTCGCCA | Start: 131 |
| Tm | 59.97 | 60.25 | End: 230 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | GAGACTCGGGCTGAAGTGAG | ACGGCTTGCGTAACGTCATA | Start: 472 |
| Tm | 59.828 | 60.109 | End: 603 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | TGGCGAAATGGAGTCGTGTT | ATGTAGACCGCGCCGTTAAT | Start: 211 |
| Tm | 60.25 | 59.896 | End: 458 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | TTGGCGAAATGGAGTCGTGT | ATGTAGACCGCGCCGTTAAT | Start: 210 |
| Tm | 60.25 | 59.896 | End: 458 |
| Size | 20 | 20 | 249 |
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