Primer Details for TRINITY_DN84801_c2_g1_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATAATTTTGCCCAAATTTCAAGGT | AGATGCCAGTCTCCGACTCA | Start: 67 |
| Tm | 59.495 | 60.324 | End: 166 |
| Size | 27 | 20 | 100 |
| Primer 2 details |
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| Sequence | ACAATAATTTTGCCCAAATTTCAAGGT | TCAAGATGCCAGTCTCCGAC | Start: 67 |
| Tm | 59.495 | 59.467 | End: 169 |
| Size | 27 | 20 | 103 |
| Primer 3 details |
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| Sequence | ACAATAATTTTGCCCAAATTTCAAGGT | CAAGATGCCAGTCTCCGACT | Start: 67 |
| Tm | 59.495 | 59.466 | End: 168 |
| Size | 27 | 20 | 102 |
| Primer 4 details |
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| Sequence | ACAATAATTTTGCCCAAATTTCAAGG | AGATGCCAGTCTCCGACTCA | Start: 67 |
| Tm | 58.116 | 60.324 | End: 166 |
| Size | 26 | 20 | 100 |
| Primer 5 details |
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| Sequence | TGCCCAAATTTCAAGGTTTTGG | GGAAATATCAAGATGCCAGTCTCC | Start: 77 |
| Tm | 58.45 | 59.241 | End: 176 |
| Size | 22 | 24 | 100 |
