ssr_primer
Primer Details for TRINITY_DN84785_c3_g3_i2 |
|---|
| (T)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTCGTTTCCCCAAGCCCT | GCACCGGTCCGAACTAGAAT | Start: 580 |
| Tm | 59.886 | 59.825 | End: 756 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
|---|
| Sequence | CATCTGGCAGAAAACGCGTC | AGGGCTTGGGGAAACGAATT | Start: 335 |
| Tm | 60.179 | 59.886 | End: 599 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
|---|
| Sequence | GTTTCCCCAAGCCCTGAAGT | GCACCGGTCCGAACTAGAAT | Start: 585 |
| Tm | 60.179 | 59.825 | End: 756 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | TTTCCCCAAGCCCTGAAGTG | GCACCGGTCCGAACTAGAAT | Start: 586 |
| Tm | 60.179 | 59.825 | End: 756 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | CATCTGGCAGAAAACGCGTC | ACTTCAGGGCTTGGGGAAAC | Start: 335 |
| Tm | 60.179 | 60.179 | End: 604 |
| Size | 20 | 20 | 270 |
(TTTA)5tctatttatcta(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTCGTTTCCCCAAGCCCT | GCACCGGTCCGAACTAGAAT | Start: 580 |
| Tm | 59.886 | 59.825 | End: 756 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
|---|
| Sequence | CATCTGGCAGAAAACGCGTC | AGGGCTTGGGGAAACGAATT | Start: 335 |
| Tm | 60.179 | 59.886 | End: 599 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
|---|
| Sequence | GTTTCCCCAAGCCCTGAAGT | GCACCGGTCCGAACTAGAAT | Start: 585 |
| Tm | 60.179 | 59.825 | End: 756 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | TTTCCCCAAGCCCTGAAGTG | GCACCGGTCCGAACTAGAAT | Start: 586 |
| Tm | 60.179 | 59.825 | End: 756 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | CATCTGGCAGAAAACGCGTC | ACTTCAGGGCTTGGGGAAAC | Start: 335 |
| Tm | 60.179 | 60.179 | End: 604 |
| Size | 20 | 20 | 270 |
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