Primer Details for TRINITY_DN84752_c11_g1_i1 |
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| (A)15tatttgattttgagacagattgtgatttttctcaattta(T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGACAGATTGTGATTTTTCTCAA | AAAGCACAAAAACGGGAGGG | Start: 57 |
| Tm | 57.194 | 59.25 | End: 177 |
| Size | 25 | 20 | 121 |
| Primer 2 details |
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| Sequence | TTGAGACAGATTGTGATTTTTCTCA | AAAGCACAAAAACGGGAGGG | Start: 56 |
| Tm | 57.194 | 59.25 | End: 177 |
| Size | 25 | 20 | 122 |
| Primer 3 details |
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| Sequence | TGAGACAGATTGTGATTTTTCTCAA | CGGGAGGGCAGGGATTTTT | Start: 57 |
| Tm | 57.194 | 60 | End: 165 |
| Size | 25 | 19 | 109 |
| Primer 4 details |
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| Sequence | TTGAGACAGATTGTGATTTTTCTCA | CGGGAGGGCAGGGATTTTT | Start: 56 |
| Tm | 57.194 | 60 | End: 165 |
| Size | 25 | 19 | 110 |
| Primer 5 details |
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| Sequence | TTGAGACAGATTGTGATTTTTCTCAA | AAAGCACAAAAACGGGAGGG | Start: 56 |
| Tm | 57.776 | 59.25 | End: 177 |
| Size | 26 | 20 | 122 |
