ssr_primer
Primer Details for TRINITY_DN84737_c2_g1_i5 |
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| (A)32caaaaaaaaacaaaaaaaacaaaaaaac(A)10caaaaaac(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCACATGTACCCTCTTCA | TCACTCAATGGAAAGTCCGGA | Start: 159 |
| Tm | 59.751 | 59.025 | End: 400 |
| Size | 20 | 21 | 242 |
| Primer 2 details |
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| Sequence | TCACACAACGGGAAGTTCGA | TCACTCAATGGAAAGTCCGGA | Start: 200 |
| Tm | 59.539 | 59.025 | End: 400 |
| Size | 20 | 21 | 201 |
| Primer 3 details |
|---|
| Sequence | ATCCGTTTGCATGAAGTCTGC | TTCGAACTTCCCGTTGTGTGA | Start: 121 |
| Tm | 59.53 | 60.135 | End: 220 |
| Size | 21 | 21 | 100 |
| Primer 4 details |
|---|
| Sequence | CCGCACATGTACCCTCTTCA | TCACTCAATGGAAAGTCCGGAA | Start: 159 |
| Tm | 59.751 | 59.629 | End: 400 |
| Size | 20 | 22 | 242 |
| Primer 5 details |
|---|
| Sequence | CCGCACATGTACCCTCTTCA | CACTCAATGGAAAGTCCGGA | Start: 159 |
| Tm | 59.751 | 57.527 | End: 399 |
| Size | 20 | 20 | 241 |
(TTTG)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCACATGTACCCTCTTCA | TCACTCAATGGAAAGTCCGGA | Start: 159 |
| Tm | 59.751 | 59.025 | End: 400 |
| Size | 20 | 21 | 242 |
| Primer 2 details |
|---|
| Sequence | TCACACAACGGGAAGTTCGA | TCACTCAATGGAAAGTCCGGA | Start: 200 |
| Tm | 59.539 | 59.025 | End: 400 |
| Size | 20 | 21 | 201 |
| Primer 3 details |
|---|
| Sequence | ATCCGTTTGCATGAAGTCTGC | TTCGAACTTCCCGTTGTGTGA | Start: 121 |
| Tm | 59.53 | 60.135 | End: 220 |
| Size | 21 | 21 | 100 |
| Primer 4 details |
|---|
| Sequence | CCGCACATGTACCCTCTTCA | TCACTCAATGGAAAGTCCGGAA | Start: 159 |
| Tm | 59.751 | 59.629 | End: 400 |
| Size | 20 | 22 | 242 |
| Primer 5 details |
|---|
| Sequence | CCGCACATGTACCCTCTTCA | CACTCAATGGAAAGTCCGGA | Start: 159 |
| Tm | 59.751 | 57.527 | End: 399 |
| Size | 20 | 20 | 241 |
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