ssr_primer
Primer Details for TRINITY_DN84495_c0_g1_i2 |
|---|
| (AAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCTCCAATCAAGCCGAGT | TCACAGGGCAAACGATCTCC | Start: 751 |
| Tm | 59.963 | 60.036 | End: 860 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTGCTCCAATCAAGCCGAGT | CGCATTTGTCCTCGAACGTC | Start: 751 |
| Tm | 59.963 | 59.907 | End: 931 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | GGAGATCGTTTGCCCTGTGA | TCGTTGTCAGGACCACAGTG | Start: 841 |
| Tm | 60.036 | 59.896 | End: 1100 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | TTTGTCAAGACCACGTGCCT | TCACAGGGCAAACGATCTCC | Start: 645 |
| Tm | 60.107 | 60.036 | End: 860 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TTTGTCAAGACCACGTGCCT | ACTCGGCTTGATTGGAGCAA | Start: 645 |
| Tm | 60.107 | 59.963 | End: 770 |
| Size | 20 | 20 | 126 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCTCCAATCAAGCCGAGT | TCACAGGGCAAACGATCTCC | Start: 751 |
| Tm | 59.963 | 60.036 | End: 860 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTGCTCCAATCAAGCCGAGT | CGCATTTGTCCTCGAACGTC | Start: 751 |
| Tm | 59.963 | 59.907 | End: 931 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | GGAGATCGTTTGCCCTGTGA | TCGTTGTCAGGACCACAGTG | Start: 841 |
| Tm | 60.036 | 59.896 | End: 1100 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | TTTGTCAAGACCACGTGCCT | TCACAGGGCAAACGATCTCC | Start: 645 |
| Tm | 60.107 | 60.036 | End: 860 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TTTGTCAAGACCACGTGCCT | ACTCGGCTTGATTGGAGCAA | Start: 645 |
| Tm | 60.107 | 59.963 | End: 770 |
| Size | 20 | 20 | 126 |
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