ssr_primer
Primer Details for TRINITY_DN84450_c6_g2_i7 |
|---|
| (CAC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGAGGTCATATGCCAGGTC | CGTTCTCCGTTTTCCTGGGA | Start: 1274 |
| Tm | 59.966 | 59.966 | End: 1501 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | TCCTGCGGGAGACAAAGTTC | TGGTGGTGGTGGTGATATGC | Start: 2165 |
| Tm | 59.965 | 60.035 | End: 2444 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
|---|
| Sequence | TCGACAATGTGGTGAGGGTG | GTCTGGGACCAGGAATGTGG | Start: 145 |
| Tm | 59.965 | 60.035 | End: 263 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
|---|
| Sequence | TCCTGCGGGAGACAAAGTTC | CTTTCGGCTGGAGAGCTCAA | Start: 2165 |
| Tm | 59.965 | 60.037 | End: 2425 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TTCGCAGTCATGTCCCAGTC | TGGTGGTGGTGGTGATATGC | Start: 2262 |
| Tm | 60.037 | 60.035 | End: 2444 |
| Size | 20 | 20 | 183 |
(A)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGAGGTCATATGCCAGGTC | CGTTCTCCGTTTTCCTGGGA | Start: 1274 |
| Tm | 59.966 | 59.966 | End: 1501 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | TCCTGCGGGAGACAAAGTTC | TGGTGGTGGTGGTGATATGC | Start: 2165 |
| Tm | 59.965 | 60.035 | End: 2444 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
|---|
| Sequence | TCGACAATGTGGTGAGGGTG | GTCTGGGACCAGGAATGTGG | Start: 145 |
| Tm | 59.965 | 60.035 | End: 263 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
|---|
| Sequence | TCCTGCGGGAGACAAAGTTC | CTTTCGGCTGGAGAGCTCAA | Start: 2165 |
| Tm | 59.965 | 60.037 | End: 2425 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TTCGCAGTCATGTCCCAGTC | TGGTGGTGGTGGTGATATGC | Start: 2262 |
| Tm | 60.037 | 60.035 | End: 2444 |
| Size | 20 | 20 | 183 |
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