ssr_primer
Primer Details for TRINITY_DN84392_c4_g2_i4 |
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| (G)18t(G)43: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGCTATGACGACGACTAA | CTGCCACTGCCCAAAACATC | Start: 122 |
| Tm | 59.971 | 60.038 | End: 275 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
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| Sequence | CTGCGCTCTCCACTGAAGAA | CACCCCTCAGCGAAAGTTCT | Start: 2990 |
| Tm | 60.038 | 59.965 | End: 3154 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
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| Sequence | CGTCCTGCACCTACTGGTTT | GCAAGTCAATCGCTGTCACC | Start: 2593 |
| Tm | 59.965 | 59.833 | End: 2699 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | CGTCCTGCACCTACTGGTTT | GGCAAGTCAATCGCTGTCAC | Start: 2593 |
| Tm | 59.965 | 59.833 | End: 2700 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
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| Sequence | ATCTTCGGCGAACACACGAT | CGGTTTGCGTCCTAGCCTTA | Start: 2463 |
| Tm | 60.109 | 60.109 | End: 2631 |
| Size | 20 | 20 | 169 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGCTATGACGACGACTAA | CTGCCACTGCCCAAAACATC | Start: 122 |
| Tm | 59.971 | 60.038 | End: 275 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | CTGCGCTCTCCACTGAAGAA | CACCCCTCAGCGAAAGTTCT | Start: 2990 |
| Tm | 60.038 | 59.965 | End: 3154 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | CGTCCTGCACCTACTGGTTT | GCAAGTCAATCGCTGTCACC | Start: 2593 |
| Tm | 59.965 | 59.833 | End: 2699 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | CGTCCTGCACCTACTGGTTT | GGCAAGTCAATCGCTGTCAC | Start: 2593 |
| Tm | 59.965 | 59.833 | End: 2700 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | ATCTTCGGCGAACACACGAT | CGGTTTGCGTCCTAGCCTTA | Start: 2463 |
| Tm | 60.109 | 60.109 | End: 2631 |
| Size | 20 | 20 | 169 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGCTATGACGACGACTAA | CTGCCACTGCCCAAAACATC | Start: 122 |
| Tm | 59.971 | 60.038 | End: 275 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | CTGCGCTCTCCACTGAAGAA | CACCCCTCAGCGAAAGTTCT | Start: 2990 |
| Tm | 60.038 | 59.965 | End: 3154 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | CGTCCTGCACCTACTGGTTT | GCAAGTCAATCGCTGTCACC | Start: 2593 |
| Tm | 59.965 | 59.833 | End: 2699 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | CGTCCTGCACCTACTGGTTT | GGCAAGTCAATCGCTGTCAC | Start: 2593 |
| Tm | 59.965 | 59.833 | End: 2700 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | ATCTTCGGCGAACACACGAT | CGGTTTGCGTCCTAGCCTTA | Start: 2463 |
| Tm | 60.109 | 60.109 | End: 2631 |
| Size | 20 | 20 | 169 |
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