ssr_primer
Primer Details for TRINITY_DN84229_c1_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCGCGGTCTTACGAGTTT | TGCTGAAGCTCCGCATTACA | Start: 557 |
| Tm | 59.967 | 60.036 | End: 815 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | AAACCGCGGTCTTACGAGTT | TGCTGAAGCTCCGCATTACA | Start: 556 |
| Tm | 59.967 | 60.036 | End: 815 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | CGGGCCCTATAAACGCTCAT | GTCGACGGGAGAGAAACGTT | Start: 1108 |
| Tm | 59.965 | 60.04 | End: 1223 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | TGAGTGGTACTGGGAGAGGG | ATGAGCGTTTATAGGGCCCG | Start: 1004 |
| Tm | 59.959 | 59.965 | End: 1127 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | TGAGTGGTACTGGGAGAGGG | GTCGACGGGAGAGAAACGTT | Start: 1004 |
| Tm | 59.959 | 60.04 | End: 1223 |
| Size | 20 | 20 | 220 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCGCGGTCTTACGAGTTT | TGCTGAAGCTCCGCATTACA | Start: 557 |
| Tm | 59.967 | 60.036 | End: 815 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | AAACCGCGGTCTTACGAGTT | TGCTGAAGCTCCGCATTACA | Start: 556 |
| Tm | 59.967 | 60.036 | End: 815 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | CGGGCCCTATAAACGCTCAT | GTCGACGGGAGAGAAACGTT | Start: 1108 |
| Tm | 59.965 | 60.04 | End: 1223 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | TGAGTGGTACTGGGAGAGGG | ATGAGCGTTTATAGGGCCCG | Start: 1004 |
| Tm | 59.959 | 59.965 | End: 1127 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | TGAGTGGTACTGGGAGAGGG | GTCGACGGGAGAGAAACGTT | Start: 1004 |
| Tm | 59.959 | 60.04 | End: 1223 |
| Size | 20 | 20 | 220 |
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