ssr_primer
Primer Details for TRINITY_DN84227_c2_g1_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATTGATTACGGGGCGAGCC | AGCAATTCAGCCATCCGTCA | Start: 426 |
| Tm | 59.966 | 60.035 | End: 690 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | ACTAGTTGGTGTTGGGCGAG | AGCAATTCAGCCATCCGTCA | Start: 466 |
| Tm | 59.965 | 60.035 | End: 690 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | TGACGGATGGCTGAATTGCT | TGGCTGTCTTGAATCTGCGT | Start: 671 |
| Tm | 60.035 | 59.965 | End: 828 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | TGCGGACGGCTCACTAAATT | GGCTCGCCCCGTAATCAATA | Start: 251 |
| Tm | 60.037 | 59.966 | End: 445 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | TGCGGACGGCTCACTAAATT | CTCGCCCAACACCAACTAGT | Start: 251 |
| Tm | 60.037 | 59.965 | End: 485 |
| Size | 20 | 20 | 235 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATTGATTACGGGGCGAGCC | AGCAATTCAGCCATCCGTCA | Start: 426 |
| Tm | 59.966 | 60.035 | End: 690 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | ACTAGTTGGTGTTGGGCGAG | AGCAATTCAGCCATCCGTCA | Start: 466 |
| Tm | 59.965 | 60.035 | End: 690 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | TGACGGATGGCTGAATTGCT | TGGCTGTCTTGAATCTGCGT | Start: 671 |
| Tm | 60.035 | 59.965 | End: 828 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | TGCGGACGGCTCACTAAATT | GGCTCGCCCCGTAATCAATA | Start: 251 |
| Tm | 60.037 | 59.966 | End: 445 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | TGCGGACGGCTCACTAAATT | CTCGCCCAACACCAACTAGT | Start: 251 |
| Tm | 60.037 | 59.965 | End: 485 |
| Size | 20 | 20 | 235 |
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