ssr_primer
Primer Details for TRINITY_DN84190_c1_g1_i7 |
|---|
| (T)26: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGCGTTCCAGTGACTCTT | TGCTGGTGGTGTGACTGTTT | Start: 2243 |
| Tm | 59.966 | 60.035 | End: 2381 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
|---|
| Sequence | CCTGCGTTCCAGTGACTCTT | GAGAAGCTGCCAATGGACCT | Start: 2243 |
| Tm | 59.966 | 60.035 | End: 2469 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | CGAAGATCCAGGCCCAAGTT | AAGAGTCACTGGAACGCAGG | Start: 2070 |
| Tm | 60.035 | 59.966 | End: 2262 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | TGATTGCCCTTGCTGATCGT | AAGAGTCACTGGAACGCAGG | Start: 2110 |
| Tm | 60.035 | 59.966 | End: 2262 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | AAACAGTCACACCACCAGCA | GAGAAGCTGCCAATGGACCT | Start: 2362 |
| Tm | 60.035 | 60.035 | End: 2469 |
| Size | 20 | 20 | 108 |
(A)14ctttaaaagttttggacagccaatcacgaattatttgaataaaacataaaagaaacagagtaacatgggtaatatt(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGCGTTCCAGTGACTCTT | TGCTGGTGGTGTGACTGTTT | Start: 2243 |
| Tm | 59.966 | 60.035 | End: 2381 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
|---|
| Sequence | CCTGCGTTCCAGTGACTCTT | GAGAAGCTGCCAATGGACCT | Start: 2243 |
| Tm | 59.966 | 60.035 | End: 2469 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | CGAAGATCCAGGCCCAAGTT | AAGAGTCACTGGAACGCAGG | Start: 2070 |
| Tm | 60.035 | 59.966 | End: 2262 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | TGATTGCCCTTGCTGATCGT | AAGAGTCACTGGAACGCAGG | Start: 2110 |
| Tm | 60.035 | 59.966 | End: 2262 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | AAACAGTCACACCACCAGCA | GAGAAGCTGCCAATGGACCT | Start: 2362 |
| Tm | 60.035 | 60.035 | End: 2469 |
| Size | 20 | 20 | 108 |
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