ssr_primer
Primer Details for TRINITY_DN84170_c3_g6_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGCTTTTGTGACGGAGGA | CTCTTCGAGCAGACACAGGG | Start: 27 |
| Tm | 60.179 | 60.109 | End: 183 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
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| Sequence | GAATCGGATGATGCGGGTCT | AGAGGCAGCGTGAATCTTGG | Start: 926 |
| Tm | 59.966 | 60.392 | End: 1059 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | TGCGGGTCTAAGTTGGCATT | AGAGGCAGCGTGAATCTTGG | Start: 937 |
| Tm | 59.962 | 60.392 | End: 1059 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
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| Sequence | CCCTTCCTCCCTTGTAACGC | CTCTTCGAGCAGACACAGGG | Start: 12 |
| Tm | 60.393 | 60.109 | End: 183 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
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| Sequence | CTTTTGTGACGGAGGACCCT | CTCTTCGAGCAGACACAGGG | Start: 31 |
| Tm | 59.603 | 60.109 | End: 183 |
| Size | 20 | 20 | 153 |
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