ssr_primer
Primer Details for TRINITY_DN84166_c0_g6_i3 |
|---|
| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTTCGCGGGGAAACAAAGC | TTGGTACTCCCGGTGATCCT | Start: 32 |
| Tm | 60.039 | 59.958 | End: 174 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CGTTCAATGCTGCCGACTTC | TAGCCAGGCCTGTCAAAGTG | Start: 998 |
| Tm | 60.179 | 59.963 | End: 1257 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | CGTTCAATGCTGCCGACTTC | GTAGCCAGGCCTGTCAAAGT | Start: 998 |
| Tm | 60.179 | 59.963 | End: 1258 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | CACTTTGACAGGCCTGGCTA | AAAATGGGTCAGTGGCACCT | Start: 1238 |
| Tm | 59.963 | 59.812 | End: 1439 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | ACTTTGACAGGCCTGGCTAC | AAAATGGGTCAGTGGCACCT | Start: 1239 |
| Tm | 59.963 | 59.812 | End: 1439 |
| Size | 20 | 20 | 201 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTTCGCGGGGAAACAAAGC | TTGGTACTCCCGGTGATCCT | Start: 32 |
| Tm | 60.039 | 59.958 | End: 174 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CGTTCAATGCTGCCGACTTC | TAGCCAGGCCTGTCAAAGTG | Start: 998 |
| Tm | 60.179 | 59.963 | End: 1257 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | CGTTCAATGCTGCCGACTTC | GTAGCCAGGCCTGTCAAAGT | Start: 998 |
| Tm | 60.179 | 59.963 | End: 1258 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | CACTTTGACAGGCCTGGCTA | AAAATGGGTCAGTGGCACCT | Start: 1238 |
| Tm | 59.963 | 59.812 | End: 1439 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | ACTTTGACAGGCCTGGCTAC | AAAATGGGTCAGTGGCACCT | Start: 1239 |
| Tm | 59.963 | 59.812 | End: 1439 |
| Size | 20 | 20 | 201 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTTCGCGGGGAAACAAAGC | TTGGTACTCCCGGTGATCCT | Start: 32 |
| Tm | 60.039 | 59.958 | End: 174 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CGTTCAATGCTGCCGACTTC | TAGCCAGGCCTGTCAAAGTG | Start: 998 |
| Tm | 60.179 | 59.963 | End: 1257 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | CGTTCAATGCTGCCGACTTC | GTAGCCAGGCCTGTCAAAGT | Start: 998 |
| Tm | 60.179 | 59.963 | End: 1258 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | CACTTTGACAGGCCTGGCTA | AAAATGGGTCAGTGGCACCT | Start: 1238 |
| Tm | 59.963 | 59.812 | End: 1439 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | ACTTTGACAGGCCTGGCTAC | AAAATGGGTCAGTGGCACCT | Start: 1239 |
| Tm | 59.963 | 59.812 | End: 1439 |
| Size | 20 | 20 | 201 |
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