Primer Details for TRINITY_DN84087_c7_g7_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCACACTTGAATTTTATGGAGA | AGCTTATGTCCTTCAATTTGTTGA | Start: 3 |
| Tm | 59.23 | 57.101 | End: 137 |
| Size | 25 | 24 | 135 |
| Primer 2 details |
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| Sequence | ACTGCACACTTGAATTTTATGGAG | AGCTTATGTCCTTCAATTTGTTGA | Start: 3 |
| Tm | 57.983 | 57.101 | End: 137 |
| Size | 24 | 24 | 135 |
| Primer 3 details |
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| Sequence | ACTGCACACTTGAATTTTATGGAGA | AGCTTATGTCCTTCAATTTGTTGAA | Start: 3 |
| Tm | 59.23 | 57.711 | End: 137 |
| Size | 25 | 25 | 135 |
| Primer 4 details |
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| Sequence | ACTGCACACTTGAATTTTATGGAGA | AAGCTTATGTCCTTCAATTTGTTGA | Start: 3 |
| Tm | 59.23 | 57.711 | End: 138 |
| Size | 25 | 25 | 136 |
| Primer 5 details |
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| Sequence | CTGCACACTTGAATTTTATGGAGA | AGCTTATGTCCTTCAATTTGTTGA | Start: 4 |
| Tm | 57.742 | 57.101 | End: 137 |
| Size | 24 | 24 | 134 |
