ssr_primer
Primer Details for TRINITY_DN84022_c2_g3_i15 |
|---|
| (T)31: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACAAGCCAAAGAAGCCAC | CGATCACCGTCTACGATGCA | Start: 844 |
| Tm | 59.967 | 59.971 | End: 1114 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | AGCCGACCACTGTAACCAAG | CGATCACCGTCTACGATGCA | Start: 938 |
| Tm | 59.965 | 59.971 | End: 1114 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
|---|
| Sequence | AAATTCAAAGCCCACGGTGC | CTCAGTATCTCGGCCCGAAC | Start: 30 |
| Tm | 59.967 | 59.969 | End: 228 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | TTTTGCCGACTCTCCAGGTC | CTCAGTATCTCGGCCCGAAC | Start: 93 |
| Tm | 59.965 | 59.969 | End: 228 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | AGTTTTGCCGACTCTCCAGG | CTCAGTATCTCGGCCCGAAC | Start: 91 |
| Tm | 59.965 | 59.969 | End: 228 |
| Size | 20 | 20 | 138 |
(T)12(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACAAGCCAAAGAAGCCAC | CGATCACCGTCTACGATGCA | Start: 844 |
| Tm | 59.967 | 59.971 | End: 1114 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | AGCCGACCACTGTAACCAAG | CGATCACCGTCTACGATGCA | Start: 938 |
| Tm | 59.965 | 59.971 | End: 1114 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
|---|
| Sequence | AAATTCAAAGCCCACGGTGC | CTCAGTATCTCGGCCCGAAC | Start: 30 |
| Tm | 59.967 | 59.969 | End: 228 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | TTTTGCCGACTCTCCAGGTC | CTCAGTATCTCGGCCCGAAC | Start: 93 |
| Tm | 59.965 | 59.969 | End: 228 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | AGTTTTGCCGACTCTCCAGG | CTCAGTATCTCGGCCCGAAC | Start: 91 |
| Tm | 59.965 | 59.969 | End: 228 |
| Size | 20 | 20 | 138 |
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