ssr_primer
Primer Details for TRINITY_DN84014_c2_g2_i2 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCAAAGGGAGTCTCGGGG | TACCCCTGTGACGACTCGAT | Start: 394 |
| Tm | 59.821 | 60.035 | End: 564 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | GCGAACCTGGGAGTGTTTTG | TACCCCTGTGACGACTCGAT | Start: 344 |
| Tm | 59.689 | 60.035 | End: 564 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | GAGGTCGGACGGGACATTTT | CAAAACACTCCCAGGTTCGC | Start: 123 |
| Tm | 60.037 | 59.689 | End: 363 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | GAGAGGGGAGAGGGACAGAA | TACCCCTGTGACGACTCGAT | Start: 366 |
| Tm | 59.663 | 60.035 | End: 564 |
| Size | 20 | 20 | 199 |
| Primer 5 details |
|---|
| Sequence | AGAGGGGAGAGGGACAGAAG | TACCCCTGTGACGACTCGAT | Start: 367 |
| Tm | 59.662 | 60.035 | End: 564 |
| Size | 20 | 20 | 198 |
(G)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCAAAGGGAGTCTCGGGG | TACCCCTGTGACGACTCGAT | Start: 394 |
| Tm | 59.821 | 60.035 | End: 564 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | GCGAACCTGGGAGTGTTTTG | TACCCCTGTGACGACTCGAT | Start: 344 |
| Tm | 59.689 | 60.035 | End: 564 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | GAGGTCGGACGGGACATTTT | CAAAACACTCCCAGGTTCGC | Start: 123 |
| Tm | 60.037 | 59.689 | End: 363 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | GAGAGGGGAGAGGGACAGAA | TACCCCTGTGACGACTCGAT | Start: 366 |
| Tm | 59.663 | 60.035 | End: 564 |
| Size | 20 | 20 | 199 |
| Primer 5 details |
|---|
| Sequence | AGAGGGGAGAGGGACAGAAG | TACCCCTGTGACGACTCGAT | Start: 367 |
| Tm | 59.662 | 60.035 | End: 564 |
| Size | 20 | 20 | 198 |
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