ssr_primer
Primer Details for TRINITY_DN84011_c1_g3_i7 |
|---|
| (T)10gtttacatgcgtttgtttgaatttttgtttttccatccctctgttcttttagaattttcgaacagggtattcccttaaattt(A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTCTGCCAATACCCGCTT | CGATCACGGACTCCCCAAAA | Start: 2819 |
| Tm | 59.962 | 60.037 | End: 2954 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | TTGTCTGCCAATACCCGCTT | ATCACGGACTCCCCAAAACC | Start: 2819 |
| Tm | 59.962 | 59.962 | End: 2952 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | TTGTCTGCCAATACCCGCTT | CTGTTTCCAGGGCATCCACT | Start: 2819 |
| Tm | 59.962 | 59.961 | End: 3003 |
| Size | 20 | 20 | 185 |
| Primer 4 details |
|---|
| Sequence | TCCCGGCTCCCTTTTAGAGA | AAGCGGGTATTGGCAGACAA | Start: 2657 |
| Tm | 59.958 | 59.962 | End: 2838 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
|---|
| Sequence | TTGTCTGCCAATACCCGCTT | GCCACACTTGTTCGCTGTTT | Start: 2819 |
| Tm | 59.962 | 59.899 | End: 3017 |
| Size | 20 | 20 | 199 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTCTGCCAATACCCGCTT | CGATCACGGACTCCCCAAAA | Start: 2819 |
| Tm | 59.962 | 60.037 | End: 2954 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | TTGTCTGCCAATACCCGCTT | ATCACGGACTCCCCAAAACC | Start: 2819 |
| Tm | 59.962 | 59.962 | End: 2952 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | TTGTCTGCCAATACCCGCTT | CTGTTTCCAGGGCATCCACT | Start: 2819 |
| Tm | 59.962 | 59.961 | End: 3003 |
| Size | 20 | 20 | 185 |
| Primer 4 details |
|---|
| Sequence | TCCCGGCTCCCTTTTAGAGA | AAGCGGGTATTGGCAGACAA | Start: 2657 |
| Tm | 59.958 | 59.962 | End: 2838 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
|---|
| Sequence | TTGTCTGCCAATACCCGCTT | GCCACACTTGTTCGCTGTTT | Start: 2819 |
| Tm | 59.962 | 59.899 | End: 3017 |
| Size | 20 | 20 | 199 |
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