ssr_primer
Primer Details for TRINITY_DN84005_c2_g3_i3 |
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| (T)12atgaatgagcgactcttggcctttagttcttcatcgatgcgcttgattttactcagcgttttttaaca(T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAATGAGCGACTCTTGGCCT | TCACTGTGATGCCGCTCAAT | Start: 112 |
| Tm | 60.108 | 60.036 | End: 389 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
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| Sequence | CACCTAGGAAAGCGGTCGAG | ATGAGGTCTTCGCGGTGTTT | Start: 483 |
| Tm | 60.179 | 59.965 | End: 741 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | CACCTAGGAAAGCGGTCGAG | AATGAGGTCTTCGCGGTGTT | Start: 483 |
| Tm | 60.179 | 59.965 | End: 742 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | CACCTAGGAAAGCGGTCGAG | AAATGAGGTCTTCGCGGTGT | Start: 483 |
| Tm | 60.179 | 59.965 | End: 743 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | ATTGAGCGGCATCACAGTGA | CTCGACCGCTTTCCTAGGTG | Start: 370 |
| Tm | 60.036 | 60.179 | End: 502 |
| Size | 20 | 20 | 133 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAATGAGCGACTCTTGGCCT | TCACTGTGATGCCGCTCAAT | Start: 112 |
| Tm | 60.108 | 60.036 | End: 389 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | CACCTAGGAAAGCGGTCGAG | ATGAGGTCTTCGCGGTGTTT | Start: 483 |
| Tm | 60.179 | 59.965 | End: 741 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | CACCTAGGAAAGCGGTCGAG | AATGAGGTCTTCGCGGTGTT | Start: 483 |
| Tm | 60.179 | 59.965 | End: 742 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | CACCTAGGAAAGCGGTCGAG | AAATGAGGTCTTCGCGGTGT | Start: 483 |
| Tm | 60.179 | 59.965 | End: 743 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | ATTGAGCGGCATCACAGTGA | CTCGACCGCTTTCCTAGGTG | Start: 370 |
| Tm | 60.036 | 60.179 | End: 502 |
| Size | 20 | 20 | 133 |
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