ssr_primer
Primer Details for TRINITY_DN83976_c4_g2_i1 |
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| (T)11gtttctttttctttt(A)10cctttgctccacatacaattcctggtggcactgaaccaaactaataataatatgggccaaattttggtctcgcacatttctacttggattga(C)11ttttctcgggaactgggcagattttgagga(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTGGCACTGAACCAAACT | AAATCTGCCCAGTTCCCGAG | Start: 213 |
| Tm | 60.034 | 60.035 | End: 317 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
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| Sequence | TGGTGGCACTGAACCAAACT | TAGCTTTCCTGCCTTGCACA | Start: 213 |
| Tm | 60.034 | 59.89 | End: 414 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
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| Sequence | CTCGGGAACTGGGCAGATTT | TAGCTTTCCTGCCTTGCACA | Start: 298 |
| Tm | 60.035 | 59.89 | End: 414 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
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| Sequence | TGGTGGCACTGAACCAAACT | CCCAGAGAGCCCCATTTAGC | Start: 213 |
| Tm | 60.034 | 60.179 | End: 441 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
|---|
| Sequence | CTCGGGAACTGGGCAGATTT | CCCAGAGAGCCCCATTTAGC | Start: 298 |
| Tm | 60.035 | 60.179 | End: 441 |
| Size | 20 | 20 | 144 |
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