Primer Details for TRINITY_DN83974_c5_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTGTTTAGGTTGGGGGTT | ACAAAACAAAACCATAAGACCCCA | Start: 13 |
| Tm | 60.179 | 59.283 | End: 203 |
| Size | 20 | 24 | 191 |
| Primer 2 details |
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| Sequence | GGCTGTTTAGGTTGGGGGTT | CGACAAAACAAAACCATAAGACCC | Start: 13 |
| Tm | 60.179 | 59.02 | End: 205 |
| Size | 20 | 24 | 193 |
| Primer 3 details |
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| Sequence | GGCTGTTTAGGTTGGGGGTT | GACAAAACAAAACCATAAGACCCCA | Start: 13 |
| Tm | 60.179 | 60.163 | End: 204 |
| Size | 20 | 25 | 192 |
| Primer 4 details |
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| Sequence | GGCTGTTTAGGTTGGGGGTT | ACAAAACAAAACCATAAGACCCCAA | Start: 13 |
| Tm | 60.179 | 59.81 | End: 203 |
| Size | 20 | 25 | 191 |
| Primer 5 details |
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| Sequence | GGCTGTTTAGGTTGGGGGTT | TCGACAAAACAAAACCATAAGACCC | Start: 13 |
| Tm | 60.179 | 60.221 | End: 206 |
| Size | 20 | 25 | 194 |
