ssr_primer
Primer Details for TRINITY_DN83929_c4_g2_i6 |
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| (T)12g(T)10acttttgttgccaagcgattccctgctctcaaatcaacttttcggtagtttcaaagttatattcattatcttgttttttttg(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAACTCCAACTGGCGTGTCC | TGGCAGAAGTTCCGCGTAAT | Start: 975 |
| Tm | 59.966 | 60.037 | End: 1231 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AGTGCGGTCATTGATGTCGT | ACATGGGTGTCGATGGTCAC | Start: 1024 |
| Tm | 60.037 | 60.036 | End: 1272 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | AGTGCGGTCATTGATGTCGT | TGGCAGAAGTTCCGCGTAAT | Start: 1024 |
| Tm | 60.037 | 60.037 | End: 1231 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
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| Sequence | AGTGCGGTCATTGATGTCGT | CGGTTGCACACTTGACATGG | Start: 1024 |
| Tm | 60.037 | 60.04 | End: 1286 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | ACACCGTCCCAAGAGTCAAC | TTGTTGTACTGCCGATCCCC | Start: 1691 |
| Tm | 59.894 | 60.036 | End: 1829 |
| Size | 20 | 20 | 139 |
(A)15tcaaactttcta(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAACTCCAACTGGCGTGTCC | TGGCAGAAGTTCCGCGTAAT | Start: 975 |
| Tm | 59.966 | 60.037 | End: 1231 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AGTGCGGTCATTGATGTCGT | ACATGGGTGTCGATGGTCAC | Start: 1024 |
| Tm | 60.037 | 60.036 | End: 1272 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | AGTGCGGTCATTGATGTCGT | TGGCAGAAGTTCCGCGTAAT | Start: 1024 |
| Tm | 60.037 | 60.037 | End: 1231 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | AGTGCGGTCATTGATGTCGT | CGGTTGCACACTTGACATGG | Start: 1024 |
| Tm | 60.037 | 60.04 | End: 1286 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | ACACCGTCCCAAGAGTCAAC | TTGTTGTACTGCCGATCCCC | Start: 1691 |
| Tm | 59.894 | 60.036 | End: 1829 |
| Size | 20 | 20 | 139 |
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