ssr_primer
Primer Details for TRINITY_DN83925_c0_g2_i1 |
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| (G)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTGTCAGCGCAGGTAGAT | ACGCAGGAGTAGAAAAGTTGGA | Start: 108 |
| Tm | 60.109 | 59.632 | End: 250 |
| Size | 20 | 22 | 143 |
| Primer 2 details |
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| Sequence | TGTGTCAGCGCAGGTAGATG | ACGCAGGAGTAGAAAAGTTGGA | Start: 109 |
| Tm | 60.109 | 59.632 | End: 250 |
| Size | 20 | 22 | 142 |
| Primer 3 details |
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| Sequence | TGTTGGGCCTTGAGGATTGT | TGCATATCCGCAAAAGGAAGG | Start: 55 |
| Tm | 59.518 | 58.976 | End: 182 |
| Size | 20 | 21 | 128 |
| Primer 4 details |
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| Sequence | TCAGCGCAGGTAGATGCATT | ACGCAGGAGTAGAAAAGTTGGA | Start: 113 |
| Tm | 59.821 | 59.632 | End: 250 |
| Size | 20 | 22 | 138 |
| Primer 5 details |
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| Sequence | AAGTTGTTGGGCCTTGAGGA | TGCATATCCGCAAAAGGAAGG | Start: 51 |
| Tm | 59.444 | 58.976 | End: 182 |
| Size | 20 | 21 | 132 |
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