ssr_primer
Primer Details for TRINITY_DN83921_c4_g3_i2 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGTCACAAATCGACAAA | CCGAGCCGATTTGCATGATG | Start: 406 |
| Tm | 59.766 | 60.041 | End: 573 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | TTTTCCGGCGTCACAAATCG | CCGAGCCGATTTGCATGATG | Start: 401 |
| Tm | 59.764 | 60.041 | End: 573 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | CATCATGCAAATCGGCTCGG | AGTGGCCTGCATTCATTTGC | Start: 554 |
| Tm | 60.041 | 59.752 | End: 789 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | CATCATGCAAATCGGCTCGG | TTCTACTGGGCCGCGTTTAA | Start: 554 |
| Tm | 60.041 | 59.679 | End: 723 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | CGGCGTCACAAATCGACAAA | TACCGAGCCGATTTGCATGA | Start: 406 |
| Tm | 59.766 | 59.823 | End: 575 |
| Size | 20 | 20 | 170 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGTCACAAATCGACAAA | CCGAGCCGATTTGCATGATG | Start: 406 |
| Tm | 59.766 | 60.041 | End: 573 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | TTTTCCGGCGTCACAAATCG | CCGAGCCGATTTGCATGATG | Start: 401 |
| Tm | 59.764 | 60.041 | End: 573 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | CATCATGCAAATCGGCTCGG | AGTGGCCTGCATTCATTTGC | Start: 554 |
| Tm | 60.041 | 59.752 | End: 789 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | CATCATGCAAATCGGCTCGG | TTCTACTGGGCCGCGTTTAA | Start: 554 |
| Tm | 60.041 | 59.679 | End: 723 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | CGGCGTCACAAATCGACAAA | TACCGAGCCGATTTGCATGA | Start: 406 |
| Tm | 59.766 | 59.823 | End: 575 |
| Size | 20 | 20 | 170 |
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