ssr_primer
Primer Details for TRINITY_DN83920_c2_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAATTAGGCACTGGGGAGGC | AATGCCAACTCTGGTAGCCC | Start: 86 |
| Tm | 60.033 | 60.034 | End: 320 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
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| Sequence | AAATTAGGCACTGGGGAGGC | AGATGAACCCCAAGAAGCGG | Start: 86 |
| Tm | 60.033 | 60.035 | End: 341 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
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| Sequence | GTTGGGCGTGTCAAGAGAGA | GGTTGAAGCCATGAGGAGCT | Start: 967 |
| Tm | 59.966 | 60.035 | End: 1138 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
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| Sequence | GTTGGGCGTGTCAAGAGAGA | AGGTTGAAGCCATGAGGAGC | Start: 967 |
| Tm | 59.966 | 60.035 | End: 1139 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
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| Sequence | GCTCCTCATGGCTTCAACCT | CAGTTGATGGGGATGGCAGT | Start: 1120 |
| Tm | 60.035 | 60.034 | End: 1305 |
| Size | 20 | 20 | 186 |
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