ssr_primer
Primer Details for TRINITY_DN83759_c5_g1_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGCCCAATCTCAGTTTGG | GCTGGCCTAGTGGGTTACAT | Start: 137 |
| Tm | 59.195 | 59.453 | End: 256 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
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| Sequence | CAATCTCAGTTTGGCGGCG | GCTGGCCTAGTGGGTTACAT | Start: 143 |
| Tm | 59.865 | 59.453 | End: 256 |
| Size | 19 | 20 | 114 |
| Primer 3 details |
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| Sequence | ACGCCCAATCTCAGTTTGGC | GCTGGCCTAGTGGGTTACAT | Start: 138 |
| Tm | 61.244 | 59.453 | End: 256 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
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| Sequence | GACGCCCAATCTCAGTTTGG | ATACAGGGCTGGCCTAGTGG | Start: 137 |
| Tm | 59.195 | 61.057 | End: 263 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
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| Sequence | GACGCCCAATCTCAGTTTGG | GGCTGGCCTAGTGGGTTAC | Start: 137 |
| Tm | 59.195 | 59.776 | End: 257 |
| Size | 20 | 19 | 121 |
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