ssr_primer
Primer Details for TRINITY_DN83668_c1_g1_i1 |
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| (T)20aaaaaaaccg(T)20aca(T)18cc(T)46: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTCCAAGGAGACTGCCAC | GTTGCCACCACTACGTGAGA | Start: 1336 |
| Tm | 60.037 | 59.967 | End: 1538 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
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| Sequence | GACTCCAAGGAGACTGCCAC | GACCATGCAAAAGCTGGCAA | Start: 1336 |
| Tm | 60.037 | 59.966 | End: 1600 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
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| Sequence | TGGCAACCAATAGTCCGGAC | ACATCCTTGGACTTCGGCAG | Start: 1532 |
| Tm | 60.036 | 60.036 | End: 1793 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
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| Sequence | GTGGCAACCAATAGTCCGGA | ACATCCTTGGACTTCGGCAG | Start: 1531 |
| Tm | 60.036 | 60.036 | End: 1793 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
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| Sequence | GACTCCAAGGAGACTGCCAC | TCCGGACTATTGGTTGCCAC | Start: 1336 |
| Tm | 60.037 | 60.036 | End: 1550 |
| Size | 20 | 20 | 215 |
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