ssr_primer
Primer Details for TRINITY_DN83653_c0_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGCGGTTCTTATACACGG | GGACCTCTTTCCCTCTCCCT | Start: 80 |
| Tm | 59.899 | 59.957 | End: 250 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
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| Sequence | AATTTTGTGCGACCTCCCCT | GGACCTCTTTCCCTCTCCCT | Start: 42 |
| Tm | 59.888 | 59.957 | End: 250 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
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| Sequence | TCGGCGGTTCTTATACACGG | GGGCCTTTTCCACAGTCTCA | Start: 80 |
| Tm | 59.899 | 59.89 | End: 189 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | AGGGAGAGGGAAAGAGGTCC | GCCAGGTTTTTATTCCGCCC | Start: 231 |
| Tm | 59.957 | 59.825 | End: 458 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
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| Sequence | AGGGAGAGGGAAAGAGGTCC | CGCCAGGTTTTTATTCCGCC | Start: 231 |
| Tm | 59.957 | 60.179 | End: 459 |
| Size | 20 | 20 | 229 |
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