ssr_primer
Primer Details for TRINITY_DN83551_c5_g2_i5 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGGCTGGGCTGAATTCCT | CCCACAATGGTAGCCAGGAA | Start: 678 |
| Tm | 59.663 | 59.669 | End: 817 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | GCGCCATCTTGCACACAATT | AGGAATTCAGCCCAGCCATT | Start: 451 |
| Tm | 60.389 | 59.663 | End: 697 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | ATGGCTGGGCTGAATTCCTG | CCCACAATGGTAGCCAGGAA | Start: 679 |
| Tm | 60.397 | 59.669 | End: 817 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | TGAAAGAATGGCTGGGCTGA | CCCACAATGGTAGCCAGGAA | Start: 672 |
| Tm | 59.595 | 59.669 | End: 817 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | GCGCCATCTTGCACACAATT | CAGGAATTCAGCCCAGCCAT | Start: 451 |
| Tm | 60.389 | 60.397 | End: 698 |
| Size | 20 | 20 | 248 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGGCTGGGCTGAATTCCT | CCCACAATGGTAGCCAGGAA | Start: 678 |
| Tm | 59.663 | 59.669 | End: 817 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | GCGCCATCTTGCACACAATT | AGGAATTCAGCCCAGCCATT | Start: 451 |
| Tm | 60.389 | 59.663 | End: 697 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | ATGGCTGGGCTGAATTCCTG | CCCACAATGGTAGCCAGGAA | Start: 679 |
| Tm | 60.397 | 59.669 | End: 817 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | TGAAAGAATGGCTGGGCTGA | CCCACAATGGTAGCCAGGAA | Start: 672 |
| Tm | 59.595 | 59.669 | End: 817 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | GCGCCATCTTGCACACAATT | CAGGAATTCAGCCCAGCCAT | Start: 451 |
| Tm | 60.389 | 60.397 | End: 698 |
| Size | 20 | 20 | 248 |
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