ssr_primer
Primer Details for TRINITY_DN83550_c1_g2_i7 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTTCAAAGTTGCCGCTGC | TCGAGACCAACTCAGTTGCC | Start: 887 |
| Tm | 59.969 | 59.966 | End: 1016 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
|---|
| Sequence | TCTTTCAAAGTTGCCGCTGC | CGAGACCAACTCAGTTGCCT | Start: 887 |
| Tm | 59.969 | 59.966 | End: 1015 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | GCGCGGGGAAAATTTCAACT | GACGAATTTCGGGCTGATGC | Start: 155 |
| Tm | 60.039 | 59.972 | End: 430 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TCTTTCAAAGTTGCCGCTGC | GCCTTCACCTTCGACTCTCC | Start: 887 |
| Tm | 59.969 | 60.109 | End: 999 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | ATTGCTAGAGAGGAAGGCGC | ACAGTCGAGAGCTCCAGAGT | Start: 1376 |
| Tm | 59.894 | 59.962 | End: 1648 |
| Size | 20 | 20 | 273 |
(A)10ttaaaaaatgcatgtaaactagtgacaacaaaaaaatgacgaaagatgtttgtaaataaatgaagaagataaagagacaaagattttttaagactg(A)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTTCAAAGTTGCCGCTGC | TCGAGACCAACTCAGTTGCC | Start: 887 |
| Tm | 59.969 | 59.966 | End: 1016 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
|---|
| Sequence | TCTTTCAAAGTTGCCGCTGC | CGAGACCAACTCAGTTGCCT | Start: 887 |
| Tm | 59.969 | 59.966 | End: 1015 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | GCGCGGGGAAAATTTCAACT | GACGAATTTCGGGCTGATGC | Start: 155 |
| Tm | 60.039 | 59.972 | End: 430 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TCTTTCAAAGTTGCCGCTGC | GCCTTCACCTTCGACTCTCC | Start: 887 |
| Tm | 59.969 | 60.109 | End: 999 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | ATTGCTAGAGAGGAAGGCGC | ACAGTCGAGAGCTCCAGAGT | Start: 1376 |
| Tm | 59.894 | 59.962 | End: 1648 |
| Size | 20 | 20 | 273 |
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