ssr_primer
Primer Details for TRINITY_DN83506_c2_g1_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAACGGAAAGGACTCGCTC | TTGTCCATTGCTTGCTTGGC | Start: 664 |
| Tm | 60.039 | 59.966 | End: 825 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
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| Sequence | TGAACGGAAAGGACTCGCTC | CCATTGCTTGCTTGGCTGTT | Start: 664 |
| Tm | 60.039 | 59.966 | End: 821 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
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| Sequence | CGCAGTGGTTGAACGGAAAG | TTGTCCATTGCTTGCTTGGC | Start: 655 |
| Tm | 60.041 | 59.966 | End: 825 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
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| Sequence | CGCAGTGGTTGAACGGAAAG | CCATTGCTTGCTTGGCTGTT | Start: 655 |
| Tm | 60.041 | 59.966 | End: 821 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
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| Sequence | TGCTGAGGGCGAAGAAGAAG | CAGCACTTCGTCCTCATCGT | Start: 352 |
| Tm | 60.037 | 60.109 | End: 472 |
| Size | 20 | 20 | 121 |
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