ssr_primer
Primer Details for TRINITY_DN83499_c5_g3_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTCGACCGGGGAATTTAT | TTTTGGCCGGAAGATGACGA | Start: 33 |
| Tm | 59.892 | 59.964 | End: 207 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
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| Sequence | GGCTCGACCGGGGAATTTAT | AAAAACAAAATGGCGGCGGA | Start: 33 |
| Tm | 59.892 | 59.895 | End: 258 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
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| Sequence | GGCTCGACCGGGGAATTTAT | AAAACAAAATGGCGGCGGAC | Start: 33 |
| Tm | 59.892 | 60.599 | End: 257 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
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| Sequence | TGTCTAGCTCTTCCCGAGGA | AAAAACAAAATGGCGGCGGA | Start: 118 |
| Tm | 59.376 | 59.895 | End: 258 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
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| Sequence | GGCTCGACCGGGGAATTTAT | TCCTCGGGAAGAGCTAGACA | Start: 33 |
| Tm | 59.892 | 59.376 | End: 137 |
| Size | 20 | 20 | 105 |
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