ssr_primer
Primer Details for TRINITY_DN83437_c2_g2_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCATGTCGGGGAAATGT | AGTGTTTTGACTGGGGCCAA | Start: 1206 |
| Tm | 59.962 | 60.033 | End: 1375 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | ACTGCATGTCGGGGAAATGT | CTCCCACGTCAGCTTTGGAT | Start: 1206 |
| Tm | 59.962 | 60.036 | End: 1419 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | TTGGCCCCAGTCAAAACACT | GGACGGCTACATTTTTCGGC | Start: 1356 |
| Tm | 60.033 | 59.902 | End: 1476 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | ACTGCATGTCGGGGAAATGT | GGACGGCTACATTTTTCGGC | Start: 1206 |
| Tm | 59.962 | 59.902 | End: 1476 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | CGCACAACAGACTGCATGTC | AGTGTTTTGACTGGGGCCAA | Start: 1196 |
| Tm | 60.111 | 60.033 | End: 1375 |
| Size | 20 | 20 | 180 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCATGTCGGGGAAATGT | AGTGTTTTGACTGGGGCCAA | Start: 1206 |
| Tm | 59.962 | 60.033 | End: 1375 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | ACTGCATGTCGGGGAAATGT | CTCCCACGTCAGCTTTGGAT | Start: 1206 |
| Tm | 59.962 | 60.036 | End: 1419 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | TTGGCCCCAGTCAAAACACT | GGACGGCTACATTTTTCGGC | Start: 1356 |
| Tm | 60.033 | 59.902 | End: 1476 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | ACTGCATGTCGGGGAAATGT | GGACGGCTACATTTTTCGGC | Start: 1206 |
| Tm | 59.962 | 59.902 | End: 1476 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | CGCACAACAGACTGCATGTC | AGTGTTTTGACTGGGGCCAA | Start: 1196 |
| Tm | 60.111 | 60.033 | End: 1375 |
| Size | 20 | 20 | 180 |
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