ssr_primer
Primer Details for TRINITY_DN83400_c4_g1_i1 |
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| (T)30: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACTCGGTGAGCTGGAGTG | GGGAAGCCCGTGGAAAGTTA | Start: 74 |
| Tm | 59.966 | 59.963 | End: 314 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
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| Sequence | AAACTCGGTGAGCTGGAGTG | AAGCCCGTGGAAAGTTAGGG | Start: 74 |
| Tm | 59.966 | 59.962 | End: 311 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
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| Sequence | TAACTTTCCACGGGCTTCCC | GAGGTTTCCAACGCGTTTCC | Start: 295 |
| Tm | 59.963 | 60.041 | End: 532 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
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| Sequence | CCCTAACTTTCCACGGGCTT | GAGGTTTCCAACGCGTTTCC | Start: 292 |
| Tm | 59.962 | 60.041 | End: 532 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
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| Sequence | TAACTTTCCACGGGCTTCCC | GTTTCCAACGCGTTTCCCAA | Start: 295 |
| Tm | 59.963 | 59.899 | End: 529 |
| Size | 20 | 20 | 235 |
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