ssr_primer
Primer Details for TRINITY_DN83329_c0_g2_i1 |
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| (T)24acctcggacagaatgcttcaagggt(G)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAATGCTTCAAGGGTGGGG | TTGGCGTACAACAAAGCAGC | Start: 73 |
| Tm | 59.96 | 59.97 | End: 212 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
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| Sequence | AGAATGCTTCAAGGGTGGGG | AAGGCACCCCATTTCCATGT | Start: 73 |
| Tm | 59.96 | 59.883 | End: 286 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
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| Sequence | GGCTCCTCTTATGCACAGCT | AAACCAAAGCAGGACACGGA | Start: 671 |
| Tm | 59.82 | 60.107 | End: 920 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
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| Sequence | AGGCTCCTCTTATGCACAGC | AAACCAAAGCAGGACACGGA | Start: 670 |
| Tm | 59.82 | 60.107 | End: 920 |
| Size | 20 | 20 | 251 |
| Primer 5 details |
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| Sequence | AGAATGCTTCAAGGGTGGGG | GCGTACAACAAAGCAGCCTT | Start: 73 |
| Tm | 59.96 | 59.689 | End: 209 |
| Size | 20 | 20 | 137 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAATGCTTCAAGGGTGGGG | TTGGCGTACAACAAAGCAGC | Start: 73 |
| Tm | 59.96 | 59.97 | End: 212 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | AGAATGCTTCAAGGGTGGGG | AAGGCACCCCATTTCCATGT | Start: 73 |
| Tm | 59.96 | 59.883 | End: 286 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | GGCTCCTCTTATGCACAGCT | AAACCAAAGCAGGACACGGA | Start: 671 |
| Tm | 59.82 | 60.107 | End: 920 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
|---|
| Sequence | AGGCTCCTCTTATGCACAGC | AAACCAAAGCAGGACACGGA | Start: 670 |
| Tm | 59.82 | 60.107 | End: 920 |
| Size | 20 | 20 | 251 |
| Primer 5 details |
|---|
| Sequence | AGAATGCTTCAAGGGTGGGG | GCGTACAACAAAGCAGCCTT | Start: 73 |
| Tm | 59.96 | 59.689 | End: 209 |
| Size | 20 | 20 | 137 |
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