ssr_primer
Primer Details for TRINITY_DN83303_c4_g3_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGAGGCTTGTGGAAGAAGT | TCGCAAATGGGTCTCAGTCC | Start: 323 |
| Tm | 59.965 | 60.036 | End: 439 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | TGCCCTACACAAAGAGCCTG | AGGAGCACGATGCCATTTCA | Start: 1108 |
| Tm | 59.963 | 60.035 | End: 1258 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
|---|
| Sequence | CGCCCAGTATGATGGAGCAT | TCGCAAATGGGTCTCAGTCC | Start: 293 |
| Tm | 59.964 | 60.036 | End: 439 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
|---|
| Sequence | CCCCTGCACAGTTGATGACT | ACTTCTTCCACAAGCCTCGG | Start: 196 |
| Tm | 59.963 | 59.965 | End: 342 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | CCCCTGCACAGTTGATGACT | ATGCTCCATCATACTGGGCG | Start: 196 |
| Tm | 59.963 | 59.964 | End: 312 |
| Size | 20 | 20 | 117 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGAGGCTTGTGGAAGAAGT | TCGCAAATGGGTCTCAGTCC | Start: 323 |
| Tm | 59.965 | 60.036 | End: 439 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | TGCCCTACACAAAGAGCCTG | AGGAGCACGATGCCATTTCA | Start: 1108 |
| Tm | 59.963 | 60.035 | End: 1258 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
|---|
| Sequence | CGCCCAGTATGATGGAGCAT | TCGCAAATGGGTCTCAGTCC | Start: 293 |
| Tm | 59.964 | 60.036 | End: 439 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
|---|
| Sequence | CCCCTGCACAGTTGATGACT | ACTTCTTCCACAAGCCTCGG | Start: 196 |
| Tm | 59.963 | 59.965 | End: 342 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | CCCCTGCACAGTTGATGACT | ATGCTCCATCATACTGGGCG | Start: 196 |
| Tm | 59.963 | 59.964 | End: 312 |
| Size | 20 | 20 | 117 |
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