ssr_primer
Primer Details for TRINITY_DN83220_c5_g1_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGGTGTCCTACGGCGTTC | AAGTGGGTGTGACAAGGCAA | Start: 639 |
| Tm | 59.9 | 60.034 | End: 781 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
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| Sequence | GGCGTTCATTTCTGGTGCAG | AAGTGGGTGTGACAAGGCAA | Start: 652 |
| Tm | 60.11 | 60.034 | End: 781 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | TGCGTTCTCGGTGGGTTTAA | ATGGATGTCACTCGGCTCAC | Start: 25 |
| Tm | 59.894 | 59.823 | End: 127 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
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| Sequence | TTGCGTTCTCGGTGGGTTTA | ATGGATGTCACTCGGCTCAC | Start: 24 |
| Tm | 59.894 | 59.823 | End: 127 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
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| Sequence | AGCATCCACGGGTTTCCAAA | CTGCACCAGAAATGAACGCC | Start: 441 |
| Tm | 60.179 | 60.11 | End: 671 |
| Size | 20 | 20 | 231 |
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