ssr_primer
Primer Details for TRINITY_DN83174_c1_g1_i10 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTAGGTTGTCGTCGATGGCA | GTTGCCTTTCAGCCAACTGG | Start: 309 |
| Tm | 60.109 | 59.967 | End: 544 |
| Size | 20 | 20 | 236 |
| Primer 2 details |
|---|
| Sequence | AGGTTGTCGTCGATGGCAAA | GTTGCCTTTCAGCCAACTGG | Start: 311 |
| Tm | 60.25 | 59.967 | End: 544 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | CGTTGCCGGCCTTTCATAAG | TTACACACAAGCAAGGGCCT | Start: 2158 |
| Tm | 59.901 | 59.815 | End: 2356 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | AGGCCCTTGCTTGTGTGTAA | CGACGCTCTTCCGATCTTGA | Start: 2337 |
| Tm | 59.815 | 59.901 | End: 2480 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | GGATCGTTCCCTCTTCCTGC | TGCCATCGACGACAACCTAC | Start: 186 |
| Tm | 60.179 | 60.109 | End: 328 |
| Size | 20 | 20 | 143 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTAGGTTGTCGTCGATGGCA | GTTGCCTTTCAGCCAACTGG | Start: 309 |
| Tm | 60.109 | 59.967 | End: 544 |
| Size | 20 | 20 | 236 |
| Primer 2 details |
|---|
| Sequence | AGGTTGTCGTCGATGGCAAA | GTTGCCTTTCAGCCAACTGG | Start: 311 |
| Tm | 60.25 | 59.967 | End: 544 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | CGTTGCCGGCCTTTCATAAG | TTACACACAAGCAAGGGCCT | Start: 2158 |
| Tm | 59.901 | 59.815 | End: 2356 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | AGGCCCTTGCTTGTGTGTAA | CGACGCTCTTCCGATCTTGA | Start: 2337 |
| Tm | 59.815 | 59.901 | End: 2480 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | GGATCGTTCCCTCTTCCTGC | TGCCATCGACGACAACCTAC | Start: 186 |
| Tm | 60.179 | 60.109 | End: 328 |
| Size | 20 | 20 | 143 |
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