ssr_primer
Primer Details for TRINITY_DN83129_c3_g1_i3 |
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| (T)10ccaatcgccattttaccataaattgggtttttttaa(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTAGAGCCTCTGGGACTC | AAAAAGGGCCTGAAGCGTTG | Start: 100 |
| Tm | 60.033 | 59.611 | End: 330 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
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| Sequence | TTCCACTCGGAGGGTTTTCC | TGCTCACTTGTGCTGGGTAG | Start: 1027 |
| Tm | 59.601 | 59.965 | End: 1261 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | CAACGCTTCAGGCCCTTTTT | TGCGTAATCATGCGTACCGA | Start: 311 |
| Tm | 59.611 | 59.899 | End: 530 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
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| Sequence | TTCCACTCGGAGGGTTTTCC | CACGGGTCATTCTGCCTGAT | Start: 1027 |
| Tm | 59.601 | 60.108 | End: 1168 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
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| Sequence | AGAGCCTCTGGGACTCCTTT | AAAAAGGGCCTGAAGCGTTG | Start: 104 |
| Tm | 59.882 | 59.611 | End: 330 |
| Size | 20 | 20 | 227 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTAGAGCCTCTGGGACTC | AAAAAGGGCCTGAAGCGTTG | Start: 100 |
| Tm | 60.033 | 59.611 | End: 330 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | TTCCACTCGGAGGGTTTTCC | TGCTCACTTGTGCTGGGTAG | Start: 1027 |
| Tm | 59.601 | 59.965 | End: 1261 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | CAACGCTTCAGGCCCTTTTT | TGCGTAATCATGCGTACCGA | Start: 311 |
| Tm | 59.611 | 59.899 | End: 530 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | TTCCACTCGGAGGGTTTTCC | CACGGGTCATTCTGCCTGAT | Start: 1027 |
| Tm | 59.601 | 60.108 | End: 1168 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | AGAGCCTCTGGGACTCCTTT | AAAAAGGGCCTGAAGCGTTG | Start: 104 |
| Tm | 59.882 | 59.611 | End: 330 |
| Size | 20 | 20 | 227 |
(T)21: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTAGAGCCTCTGGGACTC | AAAAAGGGCCTGAAGCGTTG | Start: 100 |
| Tm | 60.033 | 59.611 | End: 330 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | TTCCACTCGGAGGGTTTTCC | TGCTCACTTGTGCTGGGTAG | Start: 1027 |
| Tm | 59.601 | 59.965 | End: 1261 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | CAACGCTTCAGGCCCTTTTT | TGCGTAATCATGCGTACCGA | Start: 311 |
| Tm | 59.611 | 59.899 | End: 530 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | TTCCACTCGGAGGGTTTTCC | CACGGGTCATTCTGCCTGAT | Start: 1027 |
| Tm | 59.601 | 60.108 | End: 1168 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | AGAGCCTCTGGGACTCCTTT | AAAAAGGGCCTGAAGCGTTG | Start: 104 |
| Tm | 59.882 | 59.611 | End: 330 |
| Size | 20 | 20 | 227 |
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