ssr_primer
Primer Details for TRINITY_DN83077_c2_g1_i12 |
|---|
| (G)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGGCAGAGAGCTGAATGT | TTCGGGGTCAGGGTAGAGAG | Start: 471 |
| Tm | 60.036 | 60.033 | End: 630 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | TTCGTGGGGAGGAAGCAATC | CATATCGCATCCATGGGGCT | Start: 1252 |
| Tm | 60.035 | 60.035 | End: 1457 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | ATCTGTGATGTCTGCGGCAA | CATATCGCATCCATGGGGCT | Start: 1269 |
| Tm | 60.036 | 60.035 | End: 1457 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | AATCTGTGATGTCTGCGGCA | CATATCGCATCCATGGGGCT | Start: 1268 |
| Tm | 60.036 | 60.035 | End: 1457 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | CACCATGCCAGCTGAAGAGA | TGCCCGATGCTTGACTTTCT | Start: 950 |
| Tm | 60.036 | 59.963 | End: 1149 |
| Size | 20 | 20 | 200 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGGCAGAGAGCTGAATGT | TTCGGGGTCAGGGTAGAGAG | Start: 471 |
| Tm | 60.036 | 60.033 | End: 630 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | TTCGTGGGGAGGAAGCAATC | CATATCGCATCCATGGGGCT | Start: 1252 |
| Tm | 60.035 | 60.035 | End: 1457 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | ATCTGTGATGTCTGCGGCAA | CATATCGCATCCATGGGGCT | Start: 1269 |
| Tm | 60.036 | 60.035 | End: 1457 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | AATCTGTGATGTCTGCGGCA | CATATCGCATCCATGGGGCT | Start: 1268 |
| Tm | 60.036 | 60.035 | End: 1457 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | CACCATGCCAGCTGAAGAGA | TGCCCGATGCTTGACTTTCT | Start: 950 |
| Tm | 60.036 | 59.963 | End: 1149 |
| Size | 20 | 20 | 200 |
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