ssr_primer
Primer Details for TRINITY_DN83022_c2_g2_i1 |
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| (T)16caaatgccatatagatgtagtcagagattttcaaacgattgaaaaaacgaaaag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCGTTTCTGCTGAATTCT | GGGCCTCTGAGCAGCTTTTA | Start: 831 |
| Tm | 59.833 | 60.035 | End: 1008 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
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| Sequence | GCGCGTTTCTGCTGAATTCT | TCATGTTGACTGCGCTGGAT | Start: 831 |
| Tm | 59.833 | 60.036 | End: 1087 |
| Size | 20 | 20 | 257 |
| Primer 3 details |
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| Sequence | TTCGAAGCTTGAGGATCCCG | TTCGGAGTAAGGGAGAGGGG | Start: 1801 |
| Tm | 59.824 | 60.032 | End: 1908 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
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| Sequence | TAAAAGCTGCTCAGAGGCCC | TGAAAGGCTGGAGGTGCTTT | Start: 989 |
| Tm | 60.035 | 59.814 | End: 1263 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
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| Sequence | TAAAAGCTGCTCAGAGGCCC | TTGAAAGGCTGGAGGTGCTT | Start: 989 |
| Tm | 60.035 | 59.814 | End: 1264 |
| Size | 20 | 20 | 276 |
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