ssr_primer
Primer Details for TRINITY_DN83001_c1_g1_i6 |
|---|
| (T)42: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTATTGGGGGCAGAACCG | TGTCCCCCTGATTTTTCGCA | Start: 1070 |
| Tm | 60.034 | 59.889 | End: 1259 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | GCATGATGCATTTGGCTGGT | CGGTTCTGCCCCCAATAGTT | Start: 834 |
| Tm | 59.823 | 60.034 | End: 1089 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | AACTATTGGGGGCAGAACCG | GCCCCTAACACAGTGGGTTT | Start: 1070 |
| Tm | 60.034 | 60.179 | End: 1179 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | TGAATGCTGCCGATGGAGAG | TGCCATCGCAGGAAGTCAAT | Start: 447 |
| Tm | 60.179 | 60.035 | End: 672 |
| Size | 20 | 20 | 226 |
| Primer 5 details |
|---|
| Sequence | TGCCGATGGAGAGGGAATTG | TGCCATCGCAGGAAGTCAAT | Start: 454 |
| Tm | 59.819 | 60.035 | End: 672 |
| Size | 20 | 20 | 219 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTATTGGGGGCAGAACCG | TGTCCCCCTGATTTTTCGCA | Start: 1070 |
| Tm | 60.034 | 59.889 | End: 1259 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | GCATGATGCATTTGGCTGGT | CGGTTCTGCCCCCAATAGTT | Start: 834 |
| Tm | 59.823 | 60.034 | End: 1089 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | AACTATTGGGGGCAGAACCG | GCCCCTAACACAGTGGGTTT | Start: 1070 |
| Tm | 60.034 | 60.179 | End: 1179 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | TGAATGCTGCCGATGGAGAG | TGCCATCGCAGGAAGTCAAT | Start: 447 |
| Tm | 60.179 | 60.035 | End: 672 |
| Size | 20 | 20 | 226 |
| Primer 5 details |
|---|
| Sequence | TGCCGATGGAGAGGGAATTG | TGCCATCGCAGGAAGTCAAT | Start: 454 |
| Tm | 59.819 | 60.035 | End: 672 |
| Size | 20 | 20 | 219 |
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