ssr_primer
Primer Details for TRINITY_DN82986_c1_g2_i3 |
|---|
| (T)26: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAACGGATCTGCGCAGAAA | AGGAAGACGGATGGCTGTTG | Start: 329 |
| Tm | 60.037 | 60.036 | End: 538 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | TCGACCACATTCAGCTGCAT | TTTCTGCGCAGATCCGTTCT | Start: 212 |
| Tm | 60.036 | 60.037 | End: 348 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | AGAACGGATCTGCGCAGAAA | GGAAGACGGATGGCTGTTGA | Start: 329 |
| Tm | 60.037 | 60.036 | End: 537 |
| Size | 20 | 20 | 209 |
| Primer 4 details |
|---|
| Sequence | TCCCTTTAGGAGCCGTCAGA | CAAATGAACCCCTCCCCTCC | Start: 1759 |
| Tm | 59.959 | 60.033 | End: 1929 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | CGGATCTGCGCAGAAAACAG | AGGAAGACGGATGGCTGTTG | Start: 333 |
| Tm | 59.904 | 60.036 | End: 538 |
| Size | 20 | 20 | 206 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAACGGATCTGCGCAGAAA | AGGAAGACGGATGGCTGTTG | Start: 329 |
| Tm | 60.037 | 60.036 | End: 538 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
|---|
| Sequence | TCGACCACATTCAGCTGCAT | TTTCTGCGCAGATCCGTTCT | Start: 212 |
| Tm | 60.036 | 60.037 | End: 348 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | AGAACGGATCTGCGCAGAAA | GGAAGACGGATGGCTGTTGA | Start: 329 |
| Tm | 60.037 | 60.036 | End: 537 |
| Size | 20 | 20 | 209 |
| Primer 4 details |
|---|
| Sequence | TCCCTTTAGGAGCCGTCAGA | CAAATGAACCCCTCCCCTCC | Start: 1759 |
| Tm | 59.959 | 60.033 | End: 1929 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | CGGATCTGCGCAGAAAACAG | AGGAAGACGGATGGCTGTTG | Start: 333 |
| Tm | 59.904 | 60.036 | End: 538 |
| Size | 20 | 20 | 206 |
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