ssr_primer
Primer Details for TRINITY_DN82982_c3_g4_i1 |
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| (A)10tacaccataaatcataattttttttacaaaaaaatccgcgttaagataccactagtttaaggataaaaaaaaac(A)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCTTGGAGGCTCCTGTCT | AGGTAGTCTGACTGTGCGAA | Start: 41 |
| Tm | 59.884 | 58.383 | End: 196 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
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| Sequence | ACTCTTGGAGGCTCCTGTCT | AGGTAGTCTGACTGTGCGAAA | Start: 41 |
| Tm | 59.884 | 59.039 | End: 196 |
| Size | 20 | 21 | 156 |
| Primer 3 details |
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| Sequence | ACTCTTGGAGGCTCCTGTCT | TCTAGGTAGTCTGACTGTGCGA | Start: 41 |
| Tm | 59.884 | 60.027 | End: 199 |
| Size | 20 | 22 | 159 |
| Primer 4 details |
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| Sequence | ACTCTTGGAGGCTCCTGTCT | CTCTAGGTAGTCTGACTGTGCG | Start: 41 |
| Tm | 59.884 | 59.647 | End: 200 |
| Size | 20 | 22 | 160 |
| Primer 5 details |
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| Sequence | ACTCTTGGAGGCTCCTGTCT | CTAGGTAGTCTGACTGTGCGA | Start: 41 |
| Tm | 59.884 | 58.643 | End: 198 |
| Size | 20 | 21 | 158 |
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