ssr_primer
Primer Details for TRINITY_DN82978_c2_g1_i10 |
|---|
| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCGGAGAAGTGGATGAAA | AGCTCAGAGGGATAAGGGCA | Start: 0 |
| Tm | 59.68 | 60.031 | End: 131 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ACGCGGAGAAGTGGATGAAA | GCTCAGAGGGATAAGGGCAA | Start: 0 |
| Tm | 59.68 | 59.16 | End: 130 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | GGCGAAATGAAAGATTCCGCA | AGCTCAGAGGGATAAGGGCA | Start: 27 |
| Tm | 59.868 | 60.031 | End: 131 |
| Size | 21 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | TGGCGAAATGAAAGATTCCGC | AGCTCAGAGGGATAAGGGCA | Start: 26 |
| Tm | 59.868 | 60.031 | End: 131 |
| Size | 21 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | ACGCGGAGAAGTGGATGAAAA | AGCTCAGAGGGATAAGGGCA | Start: 0 |
| Tm | 60.271 | 60.031 | End: 131 |
| Size | 21 | 20 | 132 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCGGAGAAGTGGATGAAA | AGCTCAGAGGGATAAGGGCA | Start: 0 |
| Tm | 59.68 | 60.031 | End: 131 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ACGCGGAGAAGTGGATGAAA | GCTCAGAGGGATAAGGGCAA | Start: 0 |
| Tm | 59.68 | 59.16 | End: 130 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | GGCGAAATGAAAGATTCCGCA | AGCTCAGAGGGATAAGGGCA | Start: 27 |
| Tm | 59.868 | 60.031 | End: 131 |
| Size | 21 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | TGGCGAAATGAAAGATTCCGC | AGCTCAGAGGGATAAGGGCA | Start: 26 |
| Tm | 59.868 | 60.031 | End: 131 |
| Size | 21 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | ACGCGGAGAAGTGGATGAAAA | AGCTCAGAGGGATAAGGGCA | Start: 0 |
| Tm | 60.271 | 60.031 | End: 131 |
| Size | 21 | 20 | 132 |
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