ssr_primer
Primer Details for TRINITY_DN82963_c0_g3_i11 |
|---|
| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATTAGCGGCGCCAAATC | AAACCAGTTTTTCCGACGCG | Start: 589 |
| Tm | 59.899 | 59.972 | End: 842 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | CAGTGCGATTTATCCCGGGA | GTGCACAGATTCTCCCGGAA | Start: 12 |
| Tm | 59.894 | 60.036 | End: 119 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
|---|
| Sequence | TCAGTGCGATTTATCCCGGG | GTGCACAGATTCTCCCGGAA | Start: 11 |
| Tm | 59.894 | 60.036 | End: 119 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | TGATGGTTCCTTGCCACCTC | TGTTGGACCACGTCGTTTCA | Start: 1382 |
| Tm | 59.962 | 60.108 | End: 1546 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GCACTAAATGACTGCGCCAG | GCAGCCGTGTCTACAGCTAT | Start: 532 |
| Tm | 59.902 | 59.897 | End: 690 |
| Size | 20 | 20 | 159 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATTAGCGGCGCCAAATC | AAACCAGTTTTTCCGACGCG | Start: 589 |
| Tm | 59.899 | 59.972 | End: 842 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | CAGTGCGATTTATCCCGGGA | GTGCACAGATTCTCCCGGAA | Start: 12 |
| Tm | 59.894 | 60.036 | End: 119 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
|---|
| Sequence | TCAGTGCGATTTATCCCGGG | GTGCACAGATTCTCCCGGAA | Start: 11 |
| Tm | 59.894 | 60.036 | End: 119 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | TGATGGTTCCTTGCCACCTC | TGTTGGACCACGTCGTTTCA | Start: 1382 |
| Tm | 59.962 | 60.108 | End: 1546 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GCACTAAATGACTGCGCCAG | GCAGCCGTGTCTACAGCTAT | Start: 532 |
| Tm | 59.902 | 59.897 | End: 690 |
| Size | 20 | 20 | 159 |
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