ssr_primer
Primer Details for TRINITY_DN82956_c6_g1_i1 |
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| (T)13gctccaagcacctttaaacgcctggaaatgataaattttcaa(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACTTGACTCACTCCGCCA | GAAGTTTGCAACGTGCCCAT | Start: 358 |
| Tm | 59.966 | 59.968 | End: 479 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
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| Sequence | GCTTGGGCTCTTTCAGGGAT | GAAGTTTGCAACGTGCCCAT | Start: 282 |
| Tm | 60.034 | 59.968 | End: 479 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | TCCTTTCTGCTAAACGCGGT | ATCCCTGAAAGAGCCCAAGC | Start: 82 |
| Tm | 59.966 | 60.034 | End: 301 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
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| Sequence | GAACGGACGGACACACATCT | CTACTTGCTCCCTTTGCGGA | Start: 720 |
| Tm | 60.039 | 60.036 | End: 876 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
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| Sequence | TTGAAAACAGCTGCTGTGGC | ATCCCTGAAAGAGCCCAAGC | Start: 57 |
| Tm | 59.897 | 60.034 | End: 301 |
| Size | 20 | 20 | 245 |
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